The Aria Fusion cell sorters (Becton Dickinson) are five-laser (355nm, 405nm, 488nm, 561nm and 640nm) with 18 simultaneous fluorescent detectors. Samples can be loaded onto the sorter in a variety of tube sizes (microfuge tubes, 12X75mm or 15ml conical centrifuge tubes). The nozzle tip sizes available for sorting are 70 microns, 85 microns, 100 microns and 130 microns. Bulk sorting from one to four populations per sample is possible. Collection tubes for one- or two-way sorting are 15ml, 12X75mm or 1.5ml microfuge tubes. For three-or four-way sorting, utilize either 12X75mm or 1.5ml microfuge collection tubes. An Automatic Cell Deposition Unit (ACDU) is available for sorting onto slides and plates (6-, 24-, 48-, 96- or 384-well plates). The ACDU can help collect a single cell or any user-specified number of cells. The Aria Fusion is equipped with temperature control for the sort sample and sort collection tubes. These sorters are contained in a biosafety cabinet.