Light Microscopy/Digital Imaging Equipment

Hyperion Helios CyTOF

The Hyperion Imaging Mass Cytometer (IMC) is part of Fluidigm's line of CyTOF systems which allows for tissue samples to be “imaged” and for the masses of metals to be quantified in relation to the tissue morphology at a resolution of 1 square micron. The advantage of the system is that an investigator can label the tissue sample with as many as 50 different metal-tagged antibodies.

For more information please contact Light Microscopy Core personnel.

Leica LMD 7000

Widefield Visual Modes

Bright field
RGB Fluorescence: Metal Halide 120V

LASER

349nm (multiple adjustment parameters; solid state LASER)

Camera

CC7000

Specimen Holders

3 Slides
Petri Dish
18well slide

Collection Devices

0.2mL tubes
0.5mL tubes
Universal Collection holder (with inserts)

Features

Two Wacom Pen Screens; can draw shapes for cutting directly on monitor
Can cut and collect multiple shapes into multiple collection devices automatically
Live-Cell-Cutting (LCC) macro
Collection tube inspection macro
Collect tissues and cells directly into extraction buffer

Objectives For Cutting

PL FL 1.25x/0.4NA (cannot use for cutting)
UVI 5x/0.12 NA
PL FL L 20x/0.4 NA Corr
PL FL L 40x/0.6 NA Corr
PL FL L 63x/0.7 NA Corr

Leica MZ10F Stereomicroscope

Brightfield and Fluorescence Imaging
Dual goosenecks for illumination
EL6000 Fluorescent Source
GFP and mCherry
10:1 zoom range
Click Stop zoom settings
8x – 80x magnification range

Imaging Features

Leica DMC 4500 Digital CCD camera, USB 3.0.
1280 x 960 pixel array
Leica LAS Software

Leica Z16 Macroscope

Objectives: 0.32xAchro,0.5xApo,1xApo, 2xApo
Zoom range; 0.57x to 9.1x
Magnification: 1xApo; 7.1x to 115x (2xApo total mag 230x)
EL6000 Fluorescent Illumination

Filter Cubes

A4 for UV, BP 470/40
L5 for GFP, BP 527/30
RFP, BP 605/75
TX2 Texas Red, 645/75

Imaging Features

Leica DMC 4500 Digital CCD camera, USB 3.0.
1280 x 960 pixel array
Leica LAS Software

Olympus BX50

Observation Methods

Brightfield
Phase Contrast
Differential Interference Contrast (DIC)
Fluorescence

Digital Imaging

Q-Imaging Retiga high resolution digital monochrome camera.

Computer and Software

PC workstation with general office software and network connections
Image Pro high-end image acquisition, processing, and analysis software

Filter Cubes

Many filter cubes available upon request.

Objectives

Many different objectives available upon request.

Prairie Ultima 2 Photon Microscope

Transmitted and Fluorescence Illumination (Exfo Xcite)
Upright Configuration
Custom Heated Stage Insert

Laser Scanning Mode

Galvo; for high quality imaging
AOD; for fast imaging, e.g., 25 fps
4 Above Stage PMT Detectors (primary)
2 Substage Detectors (secondary)

Imaging Features

Motorized stage: X, Y, and Z for optical sectioning
16 bit TIFF images
128, 256, 512, 1024, 2048 pixel resolution
Frame Averaging
Adjustable Dwell Time
Advanced Time Series and Z-Sectioning
Variable Zoom
Atlas Mosaic Imaging

Coherent Laser

Coherent Chameleon Ultra II
Fast Tuning from 680nm - 1080nm
Power output approximately 3000mW at 800nm
Capable of exciting all fluorophores in the visible spectrum

Objectives:

10x/0.3 Dry WD 10mm,
20x/1.0 W Imm (XLUMPLN) WD 2mm,
40x/IR-2/0.8 W Imm WD 3.3mm,
60x/IR-2/0.8 W Imm WD 3.3mm

Spectra

View PMT Detectors and Spectra

lm-prairie-technologies-ultima-2-photon-microscope

Zeiss AxioVert 200

Observation Methods

Brightfield
Phase Contrast
Fluorescence (SOLA LED)

Digital Imaging

Zeiss Mr3 camera (mono, 1388x1040 pixel array)

Computer / Software

Dell Optiplex 790, Intel i5, 4 GB RAM, 2 19” Monitors
Windows 7
Zen Software

Objectives

5x/0.12NA A-Plan
10x/0.3NA EC Plan-Neofluar
20x/0.4NA LD Plan-Neofluar Corr
63x/1.4NA Plan-Apo Oil Imm

The Zeiss Inverted microscope is ideal for imaging assays prepared in well plates in either brightfield, phase contrast or fluorescence imaging modes. The fluorescent spectra of the filter cubes is optimized for DAPI, GFP, Cy3 and CY5 (blue, green, red and far-red).