Observation Methods Brightfield Phase Contrast (5x only) DIC Fluorescence
Digital Imaging Cameras:
Hamamatsu EMCCD C9100-13 Monochrome Camera
Very sensitive camera for high speed fluorescence acquisition.
Back-Thinned 512x512 pixel chip, 16um x 16um pixels, 31.9fps to 404fps* acquisition speed (*with gain, binning, and sub-array), Quantum Efficiency ~95%.
Zeiss MRc5 Digital Color Camera
Camera allows 36-bit color images to be acquired in high resolution due to small pixel size and large array (3.4umpixels, 2584x1936).
- 5X/0.16NA Plan-NeoFluar Phase
- 10X/0.5NA Fluar DIC
- 20X/0.5NA PlanNeofluar
- 40X/0.95 PlanApo DIC
- 63X/1.3NA Plan-NeoFluar Multi Immersion DIC, Correction Collar (working distance 170um)
Microscope also equipped with Optovar that can increase magnification by 1.6X.
Filter Cube Spectra:
#49 Ex; G369nm, Beam Splitter; FT395, Emision; 445/50 (DAPI)
#38 Ex; BP470/40, Beam Splitter; FT495, Emission; 525/50 (eGFP)
#43 Ex; BP545/25, Beam Splitter; FT570, Emission; 605/70 (DS Red)
#50 Ex; BP640/30, Beam Splitter; FT660, Emission; 690/50 (Far Red)
Computer and Software:
Intel Xeon E5-2643 4-core (8 hyperthreads), 16GB RAM, 3.3GHz, 2 TB RAID hard drive, Windows 7x64 Ultimate. DELL 24” High-Res Flat Panel Monitor.
ZEN Blue software
Capable of automated Multi Dimensional Acquisition imaging for; Time-Lapse, Z-stacks, Tiling, Multiple Positions, and Multi-Color.
Pecon / Zeiss Incubation System
Large heated enclosure, heated stage insert, CO2 control and O2 control. Can be automated via TFT or in Zen.