Hematopoietic Tissue Biorepository
The Hematopoietic Tissue Biorepository (HTB) Core was established to help further understand all aspects of normal and aberrant hematopoiesis. Its primary goal is to support high-quality, translational and clinical research on hematological diseases and malignancies by providing biological specimens of interest to investigators at City of Hope and other affiliated research institutions.
Our Mission and Vision
City of Hope’s mission is to transform the future of health care by providing blood- and bone marrow-derived cellular products, buccal cell specimens for germline nucleic acids, normal skin cells, and all associated medical information to conduct innovative research. We streamline and standardize access to research samples from both common and rare diseases, which might otherwise take years to collect for individual projects, allowing researchers to develop novel therapies, diagnostics, and the cures of the future. Our vision is to collect and bank a comprehensive tissue panel from newly diagnosed or newly relapsed participants over the full spectrum of hematological diseases seen at City of Hope.
Meet the Team
Leadership
Core Staff
Clinical Research Assistants
- Kelly Synold
[email protected]
626-423-5109 (Pager)
626-256-4673, ext. 81808 - Jennifer Zankich-Dettor
[email protected]
626-423-5874 (Pager)
626-256-4673, ext. 84901
Physicians who want patients consented or samples collected, please contact Kelly Synold or Jennifer Zankich-Dettor.
Laboratory Members
- Tinisha McDonald
Laboratory Supervisor
[email protected]
626-218-2517, Ext. 82157 - Sheyla Hernandez Amador
Research Associate - Jannette Santana
Research Associate
Investigators who have questions or special requests regarding sample preparation, processing or storage, please contact Tinisha McDonald.
Program Manager
- Lucy Ghoda, Ph.D.
[email protected]
415-578-4153 (Mobile)
626-256-4673, ext. 83605
To receive samples from the bank or to arrange for sample collection for a research project, please contact Lucy Ghoda or a co-director.
Co-Directors
- Michelle Afkhami, M.D.
Department of Pathology Representative - Ibrahim Aldoss, M.D.
Leukemia Disease Team Representative
[email protected]
626-256-4673, ext. 82405 - Elizabeth Budde, M.D.
Immunotherapy Modality Team Representative
[email protected]
626-256-4673, ext. 82405 - Alexey Danilov, M.D., Ph.D.
Lymphoma Disease Team Representative - Flavia Pichiorri, Ph.D.
Multiple Myeloma Disease Team Representative
[email protected]
626-256-4673, ext. 84908
Contact the Team
Using the Facility
- The HTB provides specimens to investigators at City of Hope and external collaborators who have an approved protocol for the use of the specimens.
- The HTS CRAs consent willing participants on the HTS's biorepository protocols and can also consent for other approved specimen collection protocols enrolling participants with hematological malignancies. The CRAs can also assist with in-house regulatory filings for investigator protocols covered under our service.
- The HTS assists investigators in research design using patient specimens and also serves as an Honest Broker to assist investigators in identifying specimens from appropriate patient cohorts.
- The HTB CRAs provide clinical data associated with the specimens, provided those data elements are covered under an approved protocol.
- The HTS Lab de-identifies, processes, and viably cryopreserves mononuclear cells enriched from bone man-ow aspirates and peripheral blood from participants with B- and T-acute lymphoblastic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic/myeloproliferative neoplasms and other hematological diseases and conditions as well as from participants receiving immune cell products for therapy.
- The HTS Lab provides specialized processing services such as magnetic bead enrichment based on immunophenotypes.
- The HTB Lab provides specimen processing services for clinical protocols.
Please contact Lucy Ghoda, Ph.D., if you require a support letter for your grant application.
Current pricing can be found on iLab Solutions.
Equipment
These are Class II Type A/B3 biosafety cabinets with vertical laminar airflow and ultraviolet light. They provide a sterile environment when working with biological specimens, e.g., peripheral blood, bone marrow and cell lines.
Two Thermo Scientific Legend XR1 Centrifuges and one Beckman Coulter Allegra X14R Centrifuge. The centrifuges have programmable settings, temperature variations, and various conical tube size adapters.
NanoDrop One is used to measure the concentration and purity of extracted DNA and RNA from samples.
This automated cell counter plays a big part in the core's daily work. The cellometer, using a stain, counts the sample concentration and provides the sample viability.
This liquid nitrogen tank offers long-term storage for cell vials in the vapor phase.
Two freezers with multiple compartments for low-temperature storage
Two freezers with multiple compartments for low-temperature storage
The deli is used to hold daily used media, stains and kits.
The 5% CO2 incubators are mainly used to culture cell lines.
This VW-sized liquid nitrogen tank is used to store specimens procured by the HTB.
Our Publications
Qin H, Dong Z, Wang X, Cheng WA, Wen F, Xue W, Sun H, Walter M, Wei G, Smith DL, Sun X, Fei F, Xie J, Panagopoulou TI, Chen CW, Song JY, Aldoss I, Kayembe C, Sarno L, Müschen M, lnghirami GG, Forman SJ, Kwak LW. Sci Trans l Med. 2019;11(511). PMID: 31554741; PMCID: PMC7015136.
Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robinson S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sl1n J, Kulkarni P, Weng H, Huang H, Chen Z, Zhang B, Wu X. Olsen MJ, Müschen M, Marcucci G, Salgia R, Li L, Fathi AT, Li Z., Mulloy JC, Wei M, Horne D, Chen J. Cancer Cell. 2020;38(1):79-96 e11. PMID: 32531268; PMCID: PMC7363590.
Wang L, Simons DL, Lu X, Tu TY, Solomon S, Wang R, Rosario A. Avalos C, Schmolze D, Yim J, Waisman J, Lee PP. Nat lmmunol. 2019;20(9):1220-30. PMID: 31285626.
Zhang B, Nguyen LXT, Li L, Zhao D, Kumar B, Vl/u H, Lin A, Pellicano F, Hopcroft L, Su YL, Copland M, Holyoake TL, Kuo CJ, Bhatia R, Snyder DS, Ali H, Stein AS, Brewer C, Wang H, McDonald T, Swiderski P, Troadec E, Chen CC, Dorrance A, Pullarkat V, Yuan YC, Perrotti D, Cadesso N, Forman SJ, Kortylewski M, Kuo YH, Marcucci G. Nat Med.
2018;24(4):450-62. PMID: 29505034; PMCID: PMC5965294.
Zhao Y, Aldoss I, Qu C, Crawford JC, Gu Z, Allen EK, Zamora AE, Alexander TB, Wang J, Goto H, Imamura T, Akahane K. Marcucci G, Stein AS, Bhatia R, Thomas PG, Forman SJ, Mullighan CG, Roberts KG. Blood. 2021;137(4):471-84. PubMed PMID: 32881995; PubMed Central PMCID: PMC7845009.
In a given year, City of Hope conducts more than 400 clinical trials enrolling more than 6,000 patients.
City of Hope is focused on basic and clinical research in cancer, diabetes and other life-threatening diseases.
