The overall goal of RPS is to provide COH investigators with access to high-quality, timely, and cost-effective pathology services that directly support a broad range of animal and human studies, and to provide regulatory-compliant access to ethically procured human biorepository collections. RPSC is composed of the Solid Tumor Biorepository (STB), Hematopoietic Tissue Biorepository including the Regulatory Office (HTB), three methodological laboratories (Research Histology, Research Molecular Pathology, Research Cytogenetics), and the Research Pathology Informatics (RPI) resource.
Research Histology laboratory provides routine histology services including processing and embedding research tissue, sectioning, staining for both FFPE and OCT blocks, special stains, EBER ISH, antibody optimization, immunohistochemistry (IHC) including multiplex IHC staining. Expert histopathology interpretation and categorization for solid and liquid tumors with digital whole slide imaging.
Research Cytogenetics laboratory provides cytogenetic and molecular cytogenetic assays to researchers at City of Hope and outside facilities. Primary services include conventional and 24-color karyotyping for both human and mouse specimens, fluorescence in situ hybridization (FISH) assays for aneuploidy, gene tracking, and gene alterations on both archival and fresh tissue, targeted genotype/phenotype FISH assays, custom probe development for human and mouse model systems, gene integration/mapping studies, as well as high resolution genomic analyses using Thermo Fisher CytoScan and OncoScan microarrays.
Research Molecular Pathology Laboratory provides targeted DNA sequencing and data interpretation, gene expression assays (Nanostring nCounter), tissue-based digital spatial profiling (GeoMx), circulating tumor cell isolation (DEParray), digital PCR, and RNA in situ hybridization (RNAscope) services. A comprehensive list of services is available in iLab.
STB and HTB services include procurement of tissue, peripheral blood/derivates from patients and healthy volunteers, bone marrow (BM) aspirates, buccal cells and skin (for germline analysis), and feces (for microbiome studies) under umbrella tissue banking protocols and normal donor blood collection protocols. The services include collecting, processing, storing, and distributing clinically annotated biospecimens. In addition, the STB and HTB collect, de-identify and process tissues for correlative studies from patients enrolled in clinical trials. HTB services also include cryopreservation of viable mononuclear cells, immunophenotype-based cell characterization and enrichment, and isolation and expansion of mesenchymal stem/stromal cells. DNA, RNA, protein extraction services are also provided.
In conjunction with the Honest Broker group, HTB and RPI provide support for annotation and cohort identification for project requests.
Lead, Research Histology and Research Molecular Pathology
Raju K. Pillai, M.D., is an assistant clinical professor specializing in hematopathology, molecular pathology and pathology bioinformatics. He is also a member of the Toni Stephenson Lymphoma Center. Dr. Pillai's research interests include molecular pathogenesis of lymphomas and leukemias and the application of panomics technologies in diagnostic pathology.